منابع مشابه
Microdiffusion chambers permitting the oil-immersion microscopy of growing microorganisms.
There is an obvious need for a technique whereby the medium surrounding an organism under microscopy can be altered without displacing the organism from the field. For example, we are interested in observing the same organisms before, during, and after exposure to various agents. Our general solution is to confine the organisms within a microchamber separated from the well of a well-slide by a ...
متن کاملUsing a quartz paraboloid for versatile wide-field TIR microscopy with sub-nanometer localization accuracy.
Illumination based on objective-type total internal reflection (TIR) is nowadays widely used in high-performance fluorescence microscopy. However, the desirable application of such setups for dark-field imaging of scattering entities is cumbersome due to the spatial overlap of illumination and detection light, which cannot be separated spectrally. Here, we report a novel TIR approach based on a...
متن کاملImmersion Lens Microscopy of Photonic Nanostructures and Quantum Dots
We describe recent experimental and theoretical advances in immersion lens microscopy for both surface and subsurface imaging as applied to photonic nanostructures. We examine in detail the ability of sharp metal tips to enhance local optical fields for nanometer resolution microscopy and spectroscopy. Finally, we describe a new approach to nano-optics, that of combining solid immersion microsc...
متن کاملAberration compensation in aplanatic solid immersion lens microscopy.
The imaging quality of an aplanatic SIL microscope is shown to be significantly degraded by aberrations, especially when the samples have thicknesses that are more than a few micrometers thicker or thinner than the design thickness. Aberration due to the sample thickness error is modeled and compared with measurements obtained in a high numerical aperture (NA ~3.5) microscope. A technique to re...
متن کاملThermal Microscopy with a Microfabricated Solid Immersion Lens
The spatial resolution of infrared thermometry is limited by diffraction to dimensions close to the wavelength of the collected infrared radiation, typically 5 μm at room temperatures. Thermal properties variations, temperature gradients, and defects with dimensions smaller than the diffraction limit are inaccessible to far-field infrared thermometry. This work demonstrates a near-field method ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature
سال: 1878
ISSN: 0028-0836,1476-4687
DOI: 10.1038/018278d0